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Br-DMEQ

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Item #	Description/Size	Availability	Qty Break	Price	Quantity
B036-10	10 mg	5-10 business days	1	$89.00

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For Research Use Only Products

Application: Carboxylic acid derivatization for HPLC analysis

Description
Application

Chemical Name: 3-Bromomethyl-6,7-dimethoxy-1-methyl-1,2-dihydroquinoxaline-2-one
CAS: 100595-07-1

Appearance: Yellow needles
Purity: ≥96.0% (HPLC)
MW: 313.15, C₁₂H₁₃BrN₂O₃

Storage Condition: -20ºC
Shipping Condition: ambient temperature

Derivatization Reaction

Product Description
Br-DMEQ is a highly fluorescent labeling reagent for carboxylates such as fatty acids and prostaglandins. Quinoxalinone compounds are formed by a reaction between 1,2-diamino-4,5-dimethoxybenzene and pyruvic acid. Br-DMEQ also reacts with pyrimidine bases of nucleic acids. Potassium carbonate and 18-crown-6 are necessary for carboxylate labeling with Br-DMEQ. The HPLC detection limit of saturated fatty acids with 3 to 20 carbons is 0.3 to 1 femtomoles per injection. The excitation and emission wavelength of labeled materials are 370 nm and 450 nm, respectively.

DMEQ Labeling Protocol
1. Dissolve a sample with acetonitrile to prepare sample solution.
2. Mix 500 μl of the sample solution, 250 μl of 3.8 mM Br-DMEQ/acetonitrile, and 250 μl of 0.8 mM 18-crown-6/acetonitrile in a reaction vial.
3. Heat the vial at 80ºC for 20 minutes and then cool it in water bath.
4. Use the mixture for HPLC analysis to determine DMEQ-labeled compounds; excitation: 370 nm, emission: 450 nm.

References

1. M. Yamaguchi, et al., 3-Bromomethyl-6, 7-dimethoxy-1-methyl-2(1H)-quinoxalinone as a New Fluorescence Derivatization Reagent for Carboxylic Acids in High-performance Liquid Chromatography. J Chromatogr A. 1985;346:227-236.
2. M. Yamaguchi, et al., Highly Sensitive Fluorogenic Reagent for Carboxylic Acid, 3-Bromomethyl-6, 7-Dimethoxy-1-Methyl-2(1H)-Quinoxalinone, in High Performance Liquid Chromatography. Anal Sci. 1985;1:295-296.
3. M. Yamaguchi, et al., Fluorimetric High-Performance Liquid Chromatography of Prostaglandins and Its Application to Their Determination in Human Seminal Fluid. J Chromatogr. 1986;380:257-265.
4. M. Yamaguchi, et al., Highly Sensitive Determination of Free Polyunsaturated, Long-Chaine Fatty Acids in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection. Anal Biochem. 1986;155:256-261.
5. M. Yamaguchi, et al., Highly Sensitive Determination of Free Fatty Acids in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection. J Chromatogr. 1986;375:27-35.
6. M. Yamaguchi, et al., Determination of 5-Fluorouracil and 5-Fluoro-2’-Deoxyuridine in Human Serum by High Performance Liquid Chromatography with Fluorescence Detection. Anal Sci. 1987;3:75-79.
7. M. Yamaguchi, et al., 3-Bromomethyl-6, 7-methylenedioxy-1-methyl-2(1H)-quinoxalinone as a Highly Sensitive Fluorescence Derivatization Reagent for Carboxylic Acids in High-performance Liquid Chromatography. Chem Pharm Bull. 1988;36:2263-2266.
8. Y. Ohkura, High Performance Liquid Chromatography of Bioactive Substances Using Bifunctional Fluorogenic Reagents for Derivatization a Review”, Anal Sci. 1989;5:371-388.