Chemical Description: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
CAS: 2348-71-2
Appearance: yellow or yellowish-orange powder
Purity: ≥97.0% (absorbance)
Molar absorptivity: ≥8,250 (around 375 nm)
MW: 414.32, C18H16BrN5S
Storage Condition : ambient temperature, protect from light
Shipping Condition: ambient temperature
Reaction Scheme
Product Description
MTT is slightly soluble in methanol, but has very low solubility in ethanol. It barely dissolves in ether, acetone, or ethyl acetate. MTT is utilized for cell viability detection. Since MTT has a positive charge, it can pass through a cell membrane and is reduced by mitochondria to form a purple color formazan dye. Organic solvent is requried to dissovle MTT formazan dyes.
References
1. T. F. Slater, et al., Studies on Succinate-tetrazolium Reductase Systems III. Points of Coupling of Four Different Tetrazolium Salts. Biochim Biophys Acta. 1963;77:383-393.
2. T. Mosmann, et al., Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. J Immunol Methods. 1983;65:55-63.
3. H. Tada, et al., An Improved Colorimetric Assay for Interleukin 2. J Immunol Methods. 1986;93:157-165.
4. M. C. Alley, et al., Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay. Cancer Res. 1988;48:589-601.
5. E. Aoyama, et al., Determination of Selenium by Flow Injection Analysis Based on the Selenium(III)-Catalyzed Reduction of 3-(4, 5-Dimethyl-2-Thiazolyl)-2, 5-Diphenyl-2H Tetrazolium Bromide. Anal Sci. 1991;7:103-107.
6. H. Yamaue, et al., Chemosensitivity Testing with Highly Purified Fresh Human Tumor Cells with the MTT Colorimetric Assay. Eur J Cancer. 1991;27:1258-1263.