Chemical Name: N,N EBis(2-hydroxy-3-sulfopropyl)tolidine, disodium salt, and other proprietary ingredients

Appearance: colorless or slightly brown solution

Storage Condition : 0-5^oC
Shipping Condition : ambient temperature

Product Description
SAT Blue is a ready-to-use solution of SAT-3 and other ingredients. SAT Blue contains no organic solvents. SAT Blue produces an intense green dye if it is mixed with peroxidase. The maximum wavelength of oxidized SAT Blue is 675 nm (Fig. 2). SAT Blue is suitable for a microplate enzyme immunoassay based on the peroxidase activity detection mechanism. Like TMBZ, oxidized SAT-3 forms a yellow dye by the addition of 2 M sulfuric acid.

Oxidation Reaction

1. Prepare an assay plate with peroxidase activity using a peroxidase-labeled antibody or peroxidase-labeled streptavidine.
2. Wash each well 4-5 times with 200-300 μl PBST (0.05% Tween 20 PBS buffer).
3. Add 100-200 μl SAT-Blue solution to each well and incubate at room temperature or 37^oC for 15-30 min.
4. Measure the O.D. at 650 nm or 670 nm.