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MGD

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Item #	Description/Size	Availability	Qty Break	Price	Quantity
M323-12	500 mg	5-10 business days	1	$116.00

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For Research Use Only Products

Application: NO detection with ESR

Description
Application

Chemical Name: N-(Dithiocarbamoyl)-N-methyl-D-glucamine, sodium salt
CAS: 94161-07-6 (free acid)

Appearance: White crystalline powder
Purity: ≥98.0% (HPLC)
MW: 293.34, C8H16NNaO5S2

Storage Condition: 0-5ºC, protect from moisture
Shipping Condition: ambient temperature

Product Description

MGD is a highly water-soluble dithiocarbamate-type chelator that generates with complexes many transitional method such as Fe and Cu. The diethyldithiocarbamate-Fe²⁺ complex has been used for NO detection by electron spin resonance (ESR). However, the poor solubility of this carbamate in an aqueous solution limits its application. Dr. Lai and others improved the technique using a water-soluble dithiocarbamate- Fe²⁺ complex, MGD-Fe²⁺. They successfully detected in vivo NO of a nitroprusside-injected mouse and NO generated by an LPS injection using in vivo ESR. The MGD-Fe²⁺ complex is capable of NO detection under physiological conditions, and dissolved oxygen in the solution does not interfere with NO detection.

Fe Complex formation

Preparation of Fe(II)-MGD Complex
1. Dissolve 278 mg FeSO₄, 7H₂O (ferrous sulfate heptahydrate) with 20 ml water^a) to prepare 50 mM FeSO₄ solution.^b)
2. Dissolve 147 mg MGD with 10 ml water^a) to prepare 50 mM MGD solution.
3. Mix 1 ml MGD solution with 8.8 ml buffer solution^a) (pH 7 or higher) and then add 200 μl FeSO₄ solution prior to use.^c)

^a) Purge any dissolved oxygen in the water or buffer by nitrogen gas bubbling for at least 30 minutes prior to dissolving FeSO₄.
^b) The FeSO₄ solution can be stored at -20ºC for at least 2 months.
^c)Fe(II)-DTCS is colorless. If the solution is brown, Fe(III)-DTCS may have formed because of dissolved oxygen in the solution. However, the brown
solution can still be used for NO trapping.

Preparation of NO-Fe(II)-MGD Complex
1. Under argon gas flow, add 200 μl FeSO₄ solution to 9.4 ml buffer solution (pH 7 or higher). Then introduce NO into the solution by bubbling NO gas through a glass capillary for 15 minutes.
2. Add 400 μl MGD solution to the FeSO₄ solution and continue to introduce NO by bubbling for another 5 minutes.
3. Remove excess NO with argon gas bubbling for 5 minutes and store at -20ºC. The NO-Fe(II)-MGD solution can be stored at -20ºC for at least 2 months in oxygen-free conditions.

References
1. L. A. Shinobu, et al., Sodium N-Methyl-D-glucamine dithiocarbamate and Cadmium in toxication. Acta Pharmacol Toxicol. 1984;54:189-194.
2. A. Komarov, et al., In vivo Spin Trapping of Nitric Oxide in Mice. Biochem Biophys Res Commun. 1993;195:1191-1198.
3. C. S. Lai, et al., Spin Trapping of Nitric Oxide Produced in vivo in Septic-shock Mice. FEBS Lett. 1994;345:120-124.
4. P. Giboreau, et al., Procedure for the Preparation of Pure Dithiocarbamates. J Org Chem. 1994;59:1205-1207.
5. B. Kalyanaraman, Detectionof Nitric Oxide by Electron Spin Resonance in Chemical, Photochemical, Cellular, Physiological, and Pathophysiological Systems. Methods Enzymol. 1996;268:168-187.
6. H. Yokoyama, et al., In vivo ESR-CT Imaging of the Liver in Mice Receiving Subcutenous Injection of Nitric Oxide-Bound Iron Complex. Magn Reson Imaging. 1997;15:249-253.
7. V. D. Mikoian, et al., Hydrophobic and Hydrophylic Fe²⁺ Complexes with Derivatives of Dithiocarbamates as a Traps of Nitric Oxide in Mouse Organism. BIOFIZIKA. 1997;42:490-501.