Chemical Name: Rhodamine 123, 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester, hydrochloride [cas# : 62669-70-9]
Appearance: Red to reddish-brown powder or solid
MW: 380.82, C21H17ClN2O3
Storage Condition : 0-5ºC, protect from light
Shipping Condition : ambient temperature
Rhodamine 123 (Rh123) is cell-membrane permeable and localizes in mitochondria of viable cells to emit yellowish-green fluorescence (Fig. 1). Rh123 is utilized for staining a wide variety of cells, including plant cells and bacteria. Since there is a correlation between the amount of ATP in a cell and the fluorescence intensity of Rh123, this compound is used for the detection of intracellular ATP. Rh123 is also used in cancer research.
Fig. 1 Cell staining mechanism