Contents of the Kit
Protein G Cartridge tube............. 1 tube Washing Buffer .......... 13 ml x 1 bottle
Elution Buffer .............. 1.8 ml x 1 tube Catching Buffer .............. 1 ml x 1 tube
1.5 mL Microtube .............. 5 tubes x 2
Storage Condition : 0-5ºC
Shipping Condition : ambient temperature
Required Equipment and Materials
Microcentrifuge, 100-200 μl adjustable pipettes, voltex mixer, 0.5 ml microtubes
IgG Purification Kits are used for isolation and purification of immunoglobulin G of goat, mouse, rabbit, and other animals. The kit contains immobilized protein A or G and buffer solutions for maximum recovery of IgG. The total time to isolate and purify IgG from serum or other solutions containing IgG is about 30 min (Fig. 1). Since the support of protein A or G is silica-base gel, the volume of the retained solution on the gel after the centrifugation is very small. Therefore, all proteins or other materials that do not bind to protein A can be removed by washing twice. SDS-PAGE of purified IgG from various animals are shown in Fig. 2. Additionally, denaturing of IgG during the elution process is minimal because IgGs bound to the gel are released with a very quick process. The gel in this kit can be used repeatedly 20 times or more with an equal performance. Used gel is also stable for one year in Washing buffer at 0-5ºC .
Fig. 1 IgG isolation process
Fig. 2 SDS-PAGE of isolated immunoglobulin by using IgG Purification
Purification Kit-A and Kit-G.
WS : Whole Serum
A : Prepared by IgG Purification Kit-A
G : Prepared by IgG Purification Kit-G
Condition: 6% acrylamide gel/Tris-glucine buffer
If the IgG solution contains gelatin,before applying the solution to the kit, enzyme digestion may be required.