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Sulfo-KMUS

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Item #	Description/Size	Availability	Qty Break	Price	Quantity
S250-10	50 mg	5-10 business days	1	$323.00

*Estimated. Exact shipping date will be notified.
For Research Use Only Products

Description
Application

Chemical Name: N-(11-Maleimidoundecanoyloxy)sulfosuccinimide, sodium salt
CAS: 211236-68-9

Appearance: White or slightly yellowish-pink powder
Purity: ≥90.0% (HPLC)
MW: 480.47, C₁₉H₂₅N₂NaO₉S

Storage Condition: 0-5ºC, protect from moisture
Shipping Condition: blue ice

Cross-linking Reaction

Product Description

Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature since it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Heterobifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.

Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent

References

1. J. V. Staros, N-Hydroxysulfosuccinimide Active Esters:Bis(N-hydroxysulfosuccinimide) Esters of Two Dicarboxylic acids Are Hydrophilic, Membraneimpermeant, Protein Cross-linkers, Biochemistry, 1982, 21, 3950.
2. P. S. R. Anjaneyulu, James V. Staros, Reactions of N-Hydroxysulfosuccinimide Active Esters, Int. J. Peptide Protein Res., 1987, 30, 117.
3. Y. Fukami, K. Sato, K. Ikeda, K. Kamisango, K. Koizumi and T. Matsuno, Evidence for Autoinhibitory Regulation of the c-src Gene Product a Possible Interaction Between the Src Homology 2 Domain and Autophosphorylation Site, J. Biol. Chem., 1993, 268, 1132.