Chemical Name: N-(11-Maleimidoundecanoyloxy)succinimide
CAS: 87981-04-2
Appearance: White powder or crystalline powder
Purity: ≥90.0% (HPLC)
MW: 378.42, C19H26N2O6
Storage Condition: 0-5ºC, protect from moisture
Shipping Condition: ambient temperature
Cross-linking Reaction
Product Description
Hetero-bifunctional cross-linking reagents
have activated esters and maleimide reactive groups. These functional
groups react with amines and sulfhydryl groups of proteins,
respectively. Enzyme-labeled haptens are prepared using
hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The
cross-linking reaction requires neutral pH and mild temperature because
it is necessary to maintain enzyme activity and antibody titers in the
cross-linking reaction. Hetero-bifunctional cross-linking reagents with
3, 5, 7, or 10 linear carbon chains are available. These linear
aliphatic chains act as spacers between the two reactive sites and their
water-soluble reagents. They are more stable than the aromatic
cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a
wider pH range.
Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent