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Liposomal
Vaccine Containing DDPC
- Drug
delivery system
(DDS) has been studied by a great number of researchers
over several decades. A DDS using liposome is thought to
be one of the most sophysticated methods.
- Professor
Junzo Sunamoto (the Department of Polymer Chemistry,
Kyoto University) and his co-workeres showed the possibility
of liposomal vaccine system using an artificial boundary
lipid.[1] In this paper, it is reported
that they prepared a liposome containing an artificial boundary
lipid, 1,2- dimyristoylamido-1,2-deoxyphosphatidylcholine
(DDPC)
for direct transfer of membrane protein of BALB RVD leukemia
cells. The liposome constructed with 20 mol% of dimyristoylphosphatidylcholine
(DMPC) and 80 mol% of DDPC
showed higher membrane protein-transfer efficiency than
any other liposomes. Membrane proteins could transfer from
BALB RVD leukemia cells to this liposome with an incubation
at 37C for 1 hr.
- This
membrane protein-transferred liposome showed significantly
higher efficiency of macrophage uptake compared with other
liposomes, such as no membrane protein-transferred liposome
and the liposome contained proteins extracted with butanol.
This results supported that tumor surface antigenic proteins
(TSAPs) were involved in membrane proteins.
- They
also tested this liposome in vivo.[2]
Cytotoxic T lymphocytes (CTLs) of immunized CB6F1 mice with
TSAP-transferred liposome were largely produced by an intradermal
injection of BALB RVD leukemia cells, and the tumor growth
was effectively prevented as they expected.
- Further
information of the liposomal vaccine would be available
from their recent report.[3]
-
DDPC
is only available from Dojindo Laboratories.
References
-
J. Sunamoto, T. Noguchi, T. Sato, K. Akiyoshi, R. Shibata,
E. Nakayama and H. Shiku, "Direct transfer of tumor
surface antigenic protein (TSAP) from tumor cell to
liposome for making liposomal vaccine", J. Control.
Release, 20, 143-154 (1992).
-
R. Shibata, T. Noguchi, T. Sato, K. Akiyoshi, J. Sunamoto,
H. Shiku, and E. Nakayama, "Induction of in vitro
and in vivo antitumor response by sensitization of mice
with liposomes reconstituted crude butanol extract from
leukemia cells and transferred intermembranously with
cell surface proteins", Int. J. Cancer,
48, 434-442 (1991).
-
K. Suzuki, Y. Okumura, T. Sato, A. Oki, M. Oki, and
J. Sunamoto, "Direct transfer of blood group antigens
from human erythrocytes to liposomes", Transfus.,
(1995) in press.
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DDPC