Discover Easy Quantitative Detection of Senescent Cells with
Cellular Senescence Detection Kit - SPiDER-ßGal



Feature

•Quantitative Detection
•Living Cells Applicable
•Staining time 30 min


Quick Outline

1. Principle
2. Differences between X-Gal method and our kit
3. State of cellular senescence research
4. Markers of Senescent Cells
5. References

Price and Availability



1. Principle


SPiDER-ßGal, a fluorescent probe in the kit, possesses cell-permeability and the ability to retain in intracellular region. SPiDER-ßGal is applicable for living cells staining and it enables to capture vivid fluorescence imaging. The kit includes SPiDER-ßGal and Bafilomycin A1


Why is Bafilomycin A1 added?
Endogenous ß-galactosidase existing in living cells prevents from detecting SA-ß-Gal selectively.
Bafilomycin A1 is an inhibitor of ATPase in lysosome. pH in lysosome is kept neutral by adding Bafilomycin A1. Cellular Senescence Detection Kit - SPiDER-ßGal contains Bafilomycin A1 which allows to detect SA-ß-Gal selectively.


?Bafilomycin A1 is utilized for living cell assays only.
?Bafilomycin A1 is not used in fixed cells because intracellular pH is controlled with the buffer.



2. Difference between X-Gal method and Cellular Senescence Detection Kit - SPiDER-ßGal





3. State of Cellular Senescence Research


Apoptotic or non-apoptotic death such as apoptosis, necrosis, autophagy and senescence are important models to understand intracellular functions. In recent years, there are reports on relationship between senescence and cancer, and senescence mechanism’s involvement in the stem cell activity.



4. Markers of Senescent Cells


Multiple markers are utilized to detect cellular senescence. SA-ß-Gal is commonly used as a marker of cellular senescence. Cellular Senescence Detection Kit - SPiDER-ßGal is a suitable kit for imaging and/or quantifying SA-ß-Gal.


5. References


SPiDER-ßGal
T. Doura, M. Kamiya, F. Obata, Y. Yamaguchi, T. Y. Hiyama, T. Matsuda, A. Fukamizu, M. Noda, M. Miura, Y. Urano, “Detection of LacZ-Positive Cells in Living Tissue with Single-Cell Resolution”, Angew Chem Int Ed Engl., 2016, 55(33), 9620-4

Cancer and cellular senescence
Rafik Salama, Mahito Sadaie, Matthew Hoare and Masashi Narita, “Cellular senescence and its effector programs”, Genes & Dev., 2014, 28, 99

Stem cell and cellular senescence
Feng Q, Lu SJ, Klimanskaya I, Gomes I, Kim D, Chung Y, Honig GR, Kim KS, Lanza R., “Hemangioblastic Derivatives from Human Induced Pluripotent Stem Cells Exhibit Limited Expansion and Early Senescence””, Stem Cells, 2010, 28(4), 704.