Storage: -20 ºC

Shipping Condition: Ambient Temperature

Chemical Name: N-(Aminooxyacetyl)-N'-biotinyl-hydrazine

CAS Number: 139585-03-8

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Appearance: white powder
Purity: >95.0 % (HPLC)
Solubility: 10 mg/ml water

Aldehyde Reactive Probe (ARP) is utilized for the detection of abasic sites (AP sites, depurine/depyrimidine sites) in DNA because of its aldehyde-specific reactivity. ARP reacts with aldehyde and ketone groups, and it adds biotin to AP sites in DNA. The biotin-tagged DNA is then be detected using peroxidase-labeled avidin and oxidative chromogenic dyes. The ARP method has been used to detect less than 1 AP site in 1x10⁴ nucleotides of DNA. Dojindo offers DNA Damage Quantification Kit (Product Code: DK02) containing all the necessary reagents and components for the determination of 1 to 40 AP sites per 1x10⁵ base pairs. ARP is highly soluble in water and the stock solution can be stored at 4 ºC for one year without significant loss of reactivity.

Labeling Procedure for DNA Abasic Site
1. Prepare 10 mM ARP with water.
2. Adjust the DNA concentration to 100 µg/ml with TE buffer (pH 7.4).
3. Mix equal volumes of the DNA and ARP solutions and incubate at 37 ºC for 1 hour.
4. Isolate the ARP-labeled DNA using either ethanol precipitation or membrane filtration tube.
5. Dissolve the DNA pellet in TE to prepare 10-100 µg/ml solution.